Biological Spill Kit Instructions

Abstract: 

When a biological spill occurs

  • Alert people in the immediate area.
  • Check yourself for contamination.
  • Assess the situation: Are you wearing all of the necessary PPE to clean this spill?
  • Cover the spill and the area around the spill with absorbent material.
  • Pour freshly made 10% bleach on absorbent materials in a spiral motion, starting from the outside of the spill working your way into the center.
  • Allow a 20-minute contact period.
  • Wipe down any contaminated stationary equipment or furniture with an appropriate disinfectant.
  • Use forceps, tongs, or broom to remove sharp objects and other items; then place in appropriate container (sharps, red bag, or regular garbage if BSL-1).
  • Clean the area again with the 10% bleach solution.
  • Decontaminate (autoclave or chemical treatment) your reusable clean-up items.
  • Remove PPE and wash your hands with soap.
  • Inform laboratory personnel when the spill clean-up is complete.
  • Contact EH&S at (510) 642-3073 or bso@berkeley.edu to report the spill.
Publication date: 
September 1, 2016
Publication type: 
Program Document