Biological Spill Kit Instructions

When a biological spill occurs

• Alert people in the immediate area.
• Check yourself for contamination.
• Assess the situation: Are you wearing all of the necessary PPE to clean this spill?
• Cover the spill and the area around the spill with absorbent material.
• Pour freshly made 10% bleach on absorbent materials in a spiral motion, starting from the outside of the spill working your way into the center.
• Allow a 20-minute contact period.
• Wipe down any contaminated stationary equipment or furniture with an appropriate disinfectant.
• Use forceps, tongs, or broom to remove sharp objects and other items; then place in appropriate container (sharps, red bag, or regular garbage if BSL-1).
• Clean the area again with the 10% bleach solution.
• Decontaminate (autoclave or chemical treatment) your reusable clean-up items.
• Remove PPE and wash your hands with soap.
• Inform laboratory personnel when the spill clean-up is complete.
• Contact EH&S at (510) 642-3073 or bso@berkeley.edu to report the spill.